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KMID : 1024420160200010028
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Food Engineering Progress
2016 Volume.20 No. 1 p.28 ~ p.34
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Application of Bacteriophages for Growth Inhibition and Biofilm Reduction of Cronobactersakazakii
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Lee Young-Duck
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Abstract
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Cronobactersakazakii is a newly emerging high hazard pathogen, which causes encephalomeningitis and necrotic colitis. Recently, successful biocontrol of harmful microorganisms in several foods through the use of bacteriophages has been reported. In this study, bacteriophages were isolated from kimchi and sewages. Morphological analysis by TEM indicated that phages belonged to the Myoviridae family. In case of heat stability, KCES2 and ESP 2949-2 phages were susceptible to temperatures above 70°C. KCES2 and ESP 2949-2 phages inhibited the growth of C. sakazakii in culture broth. When KCES2 and ESP 2949-2 phages were applied to biofilm-formed C. sakazakii, C. sakazakii was efficiently reduced. Therefore, newly isolated KCES2 and ESP 2949-2 phage for C. sakazakii might effectively reduce C. sakazakii in various foods.
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KEYWORD
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C. sakazakii, bacteriophage, growth inhibition, biofilm reduction
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